Blood-Free Medium for the Rapid Growth of Pasteurella tularensis

Blood-free Medium for the Rapid Growth of Pasteurella Tularensis.

A medium composed of (in g/100 ml) Tryptose broth with thiamine (Difco), 2.6; cysteine-HCl, 0.12; glucose, 1; FeSO(4), 7H(2)O, 0.005; KCl, 0.02; histidine, 0.1; tris(hydroxymethyl)aminomethane (tris) buffer, 0.3; and agar, 1; will support rapid growth of the fully virulent SCHU-S4 strain of Pasteurella tularensis. Although the test organism grew rapidly on medium from which KCl and tris buffer ...

A growth medium without blood cells for Pasteurella tularensis.

Chemically defined medium for the growth of Pasteurella tularensis.

Chemically defined media for the growth of Pasteurella tularensis have been described by Tamura and Fleming (1949) and more recently by Giesken (1954), Mager et al. (1954), and Traub et al. (1955). Upon application of the latter medium (Traub et al.) for the growth of a virulent strain of P. tularensis, it was noted that the viable cell yields were markedly lower than yields previously obtained...

Growth and metabolism of live vaccine strain of Pasteurella tularensis.

The growth and metabolism of the live vaccine strain of Pasteurella tularensis in different media were investigated. Maximal growth was observed in a medium containing a sulfuric acid digest of casein as amino acid source. Amino acid metabolism produced considerable ammonia, and the rate of ammonia evolution was directly proportional to the growth rate. The most likely route for amino acid brea...

Succinoxidase system of Pasteurella tularensis.

Preparation of washed particles. Cell-free extracts of highly virulent P. tularensis strain Sm were prepared by sonic disintegration of cells as described by Rendina and AMills (1957). Twenty ml of undialyzed centrifuged (3000 x G for 30 min) sonic extract, containing 3.8 to 4.2 mg SN per ml, were centrifuged for 3 hr at 105,000 X G at 5 C. The supernatant was decanted and thc reddish precipita...